Journal: bioRxiv

Article Title: NOX4 inhibition promotes the remodeling of dystrophic muscle

doi: 10.1101/2022.01.08.475493

Figure Lengend Snippet: ( A ) Representative immunofluorescent images from Nox4 WT : mdx and Nox4 KO : mdx gastrocnemius (gastroc) muscles stained with anti-NOX4 antibody. Muscle fibrosis was histologically assessed in the diaphragm and gastroc muscles from 3 month-old Nox4 WT : mdx and Nox4 KO : mdx mice using picrosirius red staining (n = 4). ( B ) Representative images and ( C-D ) histological quantifications reveal no significant differences in muscle fibrosis between the groups at this age. Data are presented as box-and-whisker plots with error bars representing minimum and maximum values. Data were analyzed using unpaired, two-tailed Welch’s T-tests (α = 0.05; effect size is presented as Cohen’s d ). Scale bar represents 100 µm.

Article Snippet: Picrosirius red staining was performed as previously described ( ) following decalcification of muscle sections using Formical-2000 (StatLab).

Techniques: Muscles, Staining, Whisker Assay, Two Tailed Test

Journal: bioRxiv

Article Title: NOX4 inhibition promotes the remodeling of dystrophic muscle

doi: 10.1101/2022.01.08.475493

Figure Lengend Snippet: ( A ) Representative picrosirius red staining and image quantification of ( B ) diaphragm and ( C ) gastrocnemius muscles from 6 month-old D2.WT (n = 6), NOX4 wild-type (NOX4-WT) and knockout (NOX4-KO) littermates from a NOX4 KO line generated on the D2. mdx background (Nox4 KO : mdx ; n = 12), and D2. mdx mice that received vehicle or GK831 (GKT) treatments beginning at 3 months of age (n = 12-14). Fibrosis quantifications for 3 month-old D2. mdx mice are included to show baseline values for GKT treatment groups. Muscle function was assessed for the ( D ) diaphragm and ( E ) extensor digitorum longus (EDL) muscles of NOX4-WT (+/+) and NOX4-KO (-/-) littermates of the Nox4 KO : mdx mouse line. ( F ) Biochemical quantification of collagen content was performed on gastrocnemius muscles of vehicle and GKT-treated D2. mdx mice. Data are presented as box-and-whisker plots with error bars representing minimum and maximum values. ( D-F ) Percent values indicate the difference between mean values of the two groups. Data were analyzed using ( B-C ) ANOVA followed by Tukey post-hoc tests (α = 0.05; effect size is reported as η 2 ) or ( D-F ) unpaired, two-tailed Welch’s T-tests (α = 0.05; effect size is reported as Cohen’s d ); *p < 0.05 vs. D2.WT values; #p < 0.05 vs. 3 mo D2. mdx values; $p < 0.05 vs. respective control group values.

Article Snippet: Picrosirius red staining was performed as previously described ( ) following decalcification of muscle sections using Formical-2000 (StatLab).

Techniques: Staining, Muscles, Knock-Out, Generated, Whisker Assay, Two Tailed Test, Control

Journal: EMBO Molecular Medicine

Article Title: Immune‐mediated ECM depletion improves tumour perfusion and payload delivery

doi: 10.15252/emmm.201910923

Figure Lengend Snippet: A Quantitative PCR analysis was performed on (top) macrophages (CD45 + /CD11b + cells) isolated from 4T1 tumours treated with 5 daily injections of indicated compounds and (bottom) cultured 4T1 cells incubated overnight with indicated compounds. Hypoxanthine‐guanine phosphoribosyltransferase (HPRT) was used as a standard (mean ± SEM are shown; n = 5; *** P < 0.001 by Student's t ‐tests). B Co‐staining of tumour sections from RIP1‐Tag5 mice treated with indicated compounds for picrosirius red (PSR; counterstained with methyl green for nuclei staining), collagen I (Col‐I), heparan sulphate proteoglycan 2 (perlecan), fibronectin and ER‐TR7. Top: Micrographs are shown for an individual staining (red). Bottom: Percentage of expression for each tumour and as means ± SEM ( n = 3 mice/group, ** P < 0.01 and *** P < 0.001, Student's t ‐test) are shown. Scale bars: 50 μm.

Article Snippet: Trichome connective tissue staining, picrosirius red staining (Abcam) and TUNEL assay (Sigma) were performed as per product protocol.

Techniques: Real-time Polymerase Chain Reaction, Isolation, Cell Culture, Incubation, Staining, Expressing